Research applications
We have established assays for research exploiting animal models as well as other special purpose assays.
More specifically, we have developed i) assays for mouse hepcidin peptides in serum/plasma (Hep-1, Hep-2) and urine (Hep-2); ii) for rat hepcidin-25 in urine; and iii) for dog, cynomolgus monkey, pig and bovine serum/plasma hepcidin-25. Our flexible WCX-TOF MS approach also allows hepcidin measurements in other human body fluids and growth medium from in vitro cultured human cell lines. For more detailed information see download.
References
Renal Handling of Circulating and Renal-Synthesized Hepcidin and Its Protective Effects against Hemoglobin-Mediated Kidney Injury
van Swelm RP, Wetzels JF, Verweij VG, Laarakkers CM, Pertijs JC, van der Wijst J, Thévenod F, Masereeuw R, Swinkels DW. J Am Soc Nephrol 2016.
Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status
Staroń R, Van Swelm RP, Lipiński P, Gajowiak A, Lenartowicz M, Bednarz A, Gajewska M, Pieszka M, Laarakkers CM, Swinkels DW, Starzyński RR. PLoS One 2015;10(8):e0136695.
The effects of the anti-hepcidin Spiegelmer NOX-H94 on inflammation-induced anemia in cynomolgus monkeys
Schwoebel F, van Eijk LT, Zboralski D, Sell S, Buchner K, Maasch C, Purschke WG, Humphrey M, Zöllner S, Eulberg D, Morich F, Pickkers P, Klussmann S. Blood 2013;121(12):2311-5.
Iron supplementation in suckling piglets: how to correct iron deficiency anemia without affecting plasma hepcidin levels
Starzyński RR, Laarakkers CM, Tjalsma H, Swinkels DW, Pieszka M, Styś A, Mickiewicz M, Lipiński P. PLoS One 2013;8(5):e64022.
Mass spectrometry analysis of hepcidin peptides in experimental mouse models
Tjalsma H, Laarakkers CM, van Swelm RP, Theurl M, Theurl I, Kemna EH, van der Burgt YE, Venselaar H, Dutilh BE, Russel FG, Weiss G, Masereeuw R, Fleming RE, Swinkels DW. PLoS One 2011;6(3):e16762.
Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathways
Kartikasari AE, Roelofs R, Schaeps RM, Kemna EH, Peters WH, Swinkels DW, Tjalsma H. Biochim Biophys Acta 2008;1784(12):2029-37.
